rat anti mouse emmprin Search Results


pe rat anti-mouse emmprin  (Becton Dickinson)
90
Becton Dickinson pe rat anti-mouse emmprin
A) Confocal images of perivascular cuffs in the cerebellar white matter of CCR2:EMMP +/+ and CCR2:EMMP −/− D18 EAE showing pan-leukocytic marker, CD45 (red) encased in basement membrane delineated by laminin staining (green). Insets showing enlarged cuffs with CD45+ cells within. Scale-50μm. B) Histograms denoting average number of cuffs observed in the two groups. Data analyzed by student’s T-test. **p<0.05, N of 6 per group. C) Spinal cords were harvested from CCR2:EMMP +/+ and CCR2:EMMP −/− mice at D18 after immunization and subjected to flow cytometry: Singlet viable cells were gated on CD11b and CD45 for CD45hi CD11b+ monocyte/macrophages as shown in dot-plot for CCR2:EMMP −/− and CCR2:EMMP +/+ examples; there were few monocyte/macrophages in the spinal cord of CCR2:EMMP −/− mice. D) Analysis of % <t>EMMPRIN+</t> CCR2+ infiltrated macrophages as well as E) expression of EMMPRIN levels (MFI = mean fluorescence intensity) in <t>CCR2+</t> <t>CD11b+LY6G-cells.</t> F) Dot plot exhibiting Ly6G and CD11b staining from blood of CCR2:EMMP +/+ and CCR2:EMMP −/− D12 EAE mice which is quantified as %CD11b+ Ly6G-CD45+ cells in (G) . Histograms showing %CD11b+ Ly6G-CD45+ cells from D18 EAE in WT, CCR2:EMMP +/+ and CCR2:EMMP −/− mice. Flow plots shown in D, E, G and H were compared using one-way ANOVA with Bonferroni post-hoc test. *p<0.05, **p<0.01 and ***p<0.001. Data represented as mean ± SD.
Pe Rat Anti Mouse Emmprin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+mouse+emmprin/pe+rat+anti+mouse+emmprin/bio_rxiv__2024__08__11__607460-189-40-45
Average 90 stars, based on 1 article reviews
pe rat anti-mouse emmprin - by Bioz Stars, 2026-09
90/100 stars
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rat anti-mouse emmprin / cd147  (RayBiotech)
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Emmprin / CD147 Rat anti-Mouse Monoclonal (Unconjugated) (OX114) Antibody, (125 µg)
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Image Search Results


A) Confocal images of perivascular cuffs in the cerebellar white matter of CCR2:EMMP +/+ and CCR2:EMMP −/− D18 EAE showing pan-leukocytic marker, CD45 (red) encased in basement membrane delineated by laminin staining (green). Insets showing enlarged cuffs with CD45+ cells within. Scale-50μm. B) Histograms denoting average number of cuffs observed in the two groups. Data analyzed by student’s T-test. **p<0.05, N of 6 per group. C) Spinal cords were harvested from CCR2:EMMP +/+ and CCR2:EMMP −/− mice at D18 after immunization and subjected to flow cytometry: Singlet viable cells were gated on CD11b and CD45 for CD45hi CD11b+ monocyte/macrophages as shown in dot-plot for CCR2:EMMP −/− and CCR2:EMMP +/+ examples; there were few monocyte/macrophages in the spinal cord of CCR2:EMMP −/− mice. D) Analysis of % EMMPRIN+ CCR2+ infiltrated macrophages as well as E) expression of EMMPRIN levels (MFI = mean fluorescence intensity) in CCR2+ CD11b+LY6G-cells. F) Dot plot exhibiting Ly6G and CD11b staining from blood of CCR2:EMMP +/+ and CCR2:EMMP −/− D12 EAE mice which is quantified as %CD11b+ Ly6G-CD45+ cells in (G) . Histograms showing %CD11b+ Ly6G-CD45+ cells from D18 EAE in WT, CCR2:EMMP +/+ and CCR2:EMMP −/− mice. Flow plots shown in D, E, G and H were compared using one-way ANOVA with Bonferroni post-hoc test. *p<0.05, **p<0.01 and ***p<0.001. Data represented as mean ± SD.

Journal: bioRxiv

Article Title: EMMPRIN confers metabolic advantage for monocytes and macrophages to promote disease in a model of multiple sclerosis

doi: 10.1101/2024.08.11.607460

Figure Lengend Snippet: A) Confocal images of perivascular cuffs in the cerebellar white matter of CCR2:EMMP +/+ and CCR2:EMMP −/− D18 EAE showing pan-leukocytic marker, CD45 (red) encased in basement membrane delineated by laminin staining (green). Insets showing enlarged cuffs with CD45+ cells within. Scale-50μm. B) Histograms denoting average number of cuffs observed in the two groups. Data analyzed by student’s T-test. **p<0.05, N of 6 per group. C) Spinal cords were harvested from CCR2:EMMP +/+ and CCR2:EMMP −/− mice at D18 after immunization and subjected to flow cytometry: Singlet viable cells were gated on CD11b and CD45 for CD45hi CD11b+ monocyte/macrophages as shown in dot-plot for CCR2:EMMP −/− and CCR2:EMMP +/+ examples; there were few monocyte/macrophages in the spinal cord of CCR2:EMMP −/− mice. D) Analysis of % EMMPRIN+ CCR2+ infiltrated macrophages as well as E) expression of EMMPRIN levels (MFI = mean fluorescence intensity) in CCR2+ CD11b+LY6G-cells. F) Dot plot exhibiting Ly6G and CD11b staining from blood of CCR2:EMMP +/+ and CCR2:EMMP −/− D12 EAE mice which is quantified as %CD11b+ Ly6G-CD45+ cells in (G) . Histograms showing %CD11b+ Ly6G-CD45+ cells from D18 EAE in WT, CCR2:EMMP +/+ and CCR2:EMMP −/− mice. Flow plots shown in D, E, G and H were compared using one-way ANOVA with Bonferroni post-hoc test. *p<0.05, **p<0.01 and ***p<0.001. Data represented as mean ± SD.

Article Snippet: Cells were then washed and incubated in following antibodies for 30 minutes at 4°C in the dark: PerCP rat anti-mouse CD45 (557235, BD Biosciences, 1:50), FITC rat anti-CD11b (553310, BD Biosciences, 1:50), APC-Cy7 rat anti-mouse Ly6G (560600, BD Biosciences, 1:50), PE rat anti-mouse EMMPRIN (562676, BD Biosciences, 1:50), BUV395 rat anti-mouse CD115 (564059, BD Biosciences, 1:50), BV510 rat anti-mouse CCR2 (CD192, 747970, BD Biosciences, 1:50) and fixable viability dye eFluor 780 (65-0865-14, eBioscience).

Techniques: Marker, Membrane, Staining, Flow Cytometry, Expressing, Fluorescence